High-Throughput DNA Sequencing Service


The term DNA sequencing actually refers to the sequencing method that used to determine the arrangement of nucleotide bases (adenine, guanine, cytosine, thymine) in the DNA.

At our center we are using the Dye-Terminator Sequencing which is developed by Frederik Sanger . The term Dye-Terminator Sequencing which means that each of the four di-deoxynucleotide chain terminators are labeled with fluorescent dyes. Each of dye will reflect different color of light when it gets excited by the laser. The labeled DNA fragments are actually subjected to electrophoresis through our high-throughput ABI 3730xl Genetic Analyzer. As a result, 4 different colors are actually observed during the electrophoresis process, and the 4 different colors represent the 4 different nucleotide bases found in DNA.

 

Our facility offers high-throughput DNA sequencing services for plasmid DNA and PCR products. Sequencing reactions are performed using Applied Biosystems BigDye terminator chemistry and are run on ABI 3730XL capillary-based DNA sequencer.

 
  PCR Product / Plasmid DNA
USM RM16
Government Agencies RM22
Private Organizations RM25
  • Fast
  • Reliable Result
  • Cheap
  • Free Daily Pick Up
  • Free Re-run For Failed Reactions
  • Free Reactions For New User

Centre For Chemical Biology (CCB@USM),
1st Floor. Block B, No-10
Persiaran Bukit Jambul,
11900 Bayan Lepas,Penang
Tel: 604-6535510 Fax: 604-6535514
Email: sequencing.ccbusm@gmail.com